The new crRNA target on neo could substitute the present neo cassette with any desired DNA sequence via CRISPR–Cas-assisted HDR. Thus, successive genome editing could be performed using solely two selectable markers in a “ping-pong” fashion. We used this methodology to create a CRISPR–Cas-assisted marker recycling system (Fig.4). The site-specific DNA cleavage created by Cas12a allowed the creation of frameshift insertion and deletion mutations by non-homologous end-joining restore, which led to loss-of-function of the goal genes. Thermophila, we designed a crRNA expression cassette to target amdS, a gene that’s important for development on acetamide as the only nitrogen source, deletion of which resulted in resistance to fluoroacetamide .
Finally, robotization indirectly strengthened “bureaucratic control” by “deskilling.” Friedman identified that capitalists can adopt a divide-and-conquer approach to cope with core and marginal workers, which is official management. However, the results of robotization isn’t solely to divide and conquer workers but also to transform some core workers into marginal workers. Because robots tremendously weaken the dependence on core staff with skills, administration can use basic employees, dispatch staff, short-term workers, and summer job-takers to exchange them. The stratification contained in the labor drive is important, and the phrases of employment loved by different varieties of staff are completely different. This course of of transforming core staff into marginal workers weakens workers’ bargaining power within the workplace and places them in a more fragile labor relationship.
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