For quantitative estimation of the phyto-constituents, HP-TLC approach is extensively used because of the accuracy and simplicity of the technique. When samples are collected which differ in the space where they’re grown and the climatic conditions, they’re evaluated for various properties and phytochemical elements. Apart from chromatograms and fingerprints digital pictures, visible detection can be possible even when the amount of samples and requirements are ready in microliter concentration. Simultaneous utility of samples and requirements on the identical HP-TLC plate is feasible and because of this property, it turns into simpler through the comparative research of herbal medicine and formulations .