In the discovery domain, the identification of active compounds from the natural extract is essentially the most troublesome and difficult task. In modern discovery, the processes like isolation with preparative bio-activity don’t match the pace as they’re gradual and tedious, whereas trendy techniques choose for simple, accurate, and quick processes to be utilized. Traditional methods, which use multiple isolations, can result in lower or fully vanishing of the activity of the energetic compound. So to overcome the drawbacks, profiling of compounds with HPLC analytical technique is done in trendy discovery to establish the multiple compounds precisely as a outcome of versatility of the analytical technique . With the growing advances in science, HPTLC is becoming one of many prime options for the quality management of the natural vegetation.
In case of the non-volatile components, they should be derivatized and then the evaluation have to be carried out . Identification of parts , separation of parts, and quantification of various compounds are each volatile and non-volatile in a single analysis. It is possible to carry out the simultaneous analysis of different compounds .