The anticipated lengths of disrupted transformants of bar, ap-3 and prk-6 have been 0.eight, 2.0 and zero.8 kb, respectively, while those of the host strain was 2.zero, 1.2 and 1.2 kb, respectively. Heterokaryotic transformants confirmed two PCR bands (both of wild-type and knockout). Verification of quadruple-gene deletions of neo, alp-1, rca-1 and hcr-1 in chosen transformants with co-transformation of solely four donor-DNAs with out CRISPR expressing cassettes. Schematic of homologous recombination of goal genes mediated by each donor DNA.
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