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In order to efficiently excise the marker gene in the next spherical transformation, we used only one marker and integrated this marker gene into a single random locus of goal genes per every spherical of edits. In the primary transformation, we focused three genes including cre-1, res-1, and gh1-1 and only locus of gh1-1 was randomly selected for marker gene neo integration, which is handy for neo knockout in successive transformation. In the second transformation, we focused four genes (neo, alp-1, rca-1 and hcr-1) and only locus of alp-1 was selected for second marker gene bar integration, which is handy for bar deletion in the third transformation. In successive transformation, we engineered three genes including bar, ap-3 and prk-6 and only locus of ap-3 was selected for first marker gene neo integration, which is handy for neo deletion in the next iteration. Advisor Group is spending $65 million to improve its technology away from its legacy AIG products. End users, trainers, content material developers, administrators, help and internet hosting groups, QA and testing teams, system house owners, external suppliers and repair companions.
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